Co-expression of Extensively drug resistant (XDR) clinical isolates of Pseudomonas aeruginosa harboring FOX and MOX ampicillinase Gene

Authors

  • Beatrice Ngozi John-Onwe Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, P.M.B. 53, Nigeria https://orcid.org/0009-0006-4943-6958
  • Francis Amadi Ibiam Department of Otorhinlaryngology (ENT), Alex Ekwueme Federal University Ndufu-Alike, P.M.B. 1010, Ikwo, Ebonyi State, Nigeria https://orcid.org/0009-0003-4592-6659
  • Evangeline Chinyere Udenweze Department of Applied Microbiology and Brewing, Faculty of Bioscience, Nnamdi Azikiwe University, Awka, Anambra, P. M. B. 5025, Nigeria https://orcid.org/0009-0003-5603-6491
  • Chidinma Stacy Iroha Department of Pharmacy, Alex Ekwueme Federal University Teaching hospital Abakaliki, Ebonyi State, P. M. B. 102, Nigeria https://orcid.org/0000-0003-3433-8942
  • Christiana Inuaesiet Edemekong Department of Biotechnology, Faculty of Pure and Applied Science, Federal College of Dental Technology and Therapy, Trans-Ekulu, P.M.B. 01473, Enugu, Nigeria. https://orcid.org/0009-0002-0705-3650
  • Ikemesit Udeme Peter Department of Public Health, Faculty of Health Technology and Engineering, Federal College of Dental Technology and Therapy, Trans-Ekulu, P.M.B. 01473, Enugu, Nigeria. https://orcid.org/0000-0001-9890-8786
  • Ifeanyichukwu Romanus Iroha Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, P.M.B. 53, Nigeria https://orcid.org/0000-0002-8198-3955

DOI:

https://doi.org/10.22270/ijmspr.v9i3.76

Keywords:

XDR, Pseudomonas aeruginosa, FOX, MOX ampicillinase

Abstract

This study determines the Co-expression of clinical isolates of XDR Pseudomonas aeruginosa harboring FOX and MOX ampicillinase Gene. A total of five (500) hundred “Clean catch” midstream urine and wound samples collected from patients at a tertiary healthcare institution in Ebonyi State Nigeria were analyzed using standard microbiological techniques. Determination of XDR Pseudomonas aeruginosa isolates was by Kirby-Bauer disc diffusion method. Molecular characterization of FOX and MOX ampicillinase resistant genes were done by PCR using specific primers. In total, the Pseudomonas aeruginosa accounted for 22.6 %. XDR Pseudomonas aeruginosa accounted for 17. 8 % and 25.3 % in Wound and urine samples respectively. All the XDR Pseudomonas aeruginosa harbored FOX and MOX ampicillinase resistant Gene. The high frequency of XDR Pseudomonas aeruginosa in our study is very worrisome and could have significant public health impact such as treatment failures, and possibly death, if not properly managed. The solutions to this crisis are to allocate more resources to basic and clinical research and to infection control and antimicrobial stewardship, to develop new antimicrobials, and to optimize the use of those that are currently available.

Keywords: XDR, Pseudomonas aeruginosa, FOX, MOX ampicillinase

Author Biographies

Beatrice Ngozi John-Onwe , Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, P.M.B. 53, Nigeria

Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, P.M.B. 53, Nigeria

Francis Amadi Ibiam, Department of Otorhinlaryngology (ENT), Alex Ekwueme Federal University Ndufu-Alike, P.M.B. 1010, Ikwo, Ebonyi State, Nigeria

Department of Otorhinlaryngology (ENT), Alex Ekwueme Federal University Ndufu-Alike, P.M.B. 1010, Ikwo, Ebonyi State, Nigeria

Evangeline Chinyere Udenweze, Department of Applied Microbiology and Brewing, Faculty of Bioscience, Nnamdi Azikiwe University, Awka, Anambra, P. M. B. 5025, Nigeria

Department of Applied Microbiology and Brewing, Faculty of Bioscience, Nnamdi Azikiwe University, Awka, Anambra, P. M. B. 5025, Nigeria

Chidinma Stacy Iroha, Department of Pharmacy, Alex Ekwueme Federal University Teaching hospital Abakaliki, Ebonyi State, P. M. B. 102, Nigeria

Department of Pharmacy, Alex Ekwueme Federal University Teaching hospital Abakaliki, Ebonyi State, P. M. B. 102, Nigeria

Christiana Inuaesiet Edemekong, Department of Biotechnology, Faculty of Pure and Applied Science, Federal College of Dental Technology and Therapy, Trans-Ekulu, P.M.B. 01473, Enugu, Nigeria.

Department of Biotechnology, Faculty of Pure and Applied Science, Federal College of Dental Technology and Therapy, Trans-Ekulu, P.M.B. 01473, Enugu, Nigeria.

Ikemesit Udeme Peter, Department of Public Health, Faculty of Health Technology and Engineering, Federal College of Dental Technology and Therapy, Trans-Ekulu, P.M.B. 01473, Enugu, Nigeria.

Department of Public Health, Faculty of Health Technology and Engineering, Federal College of Dental Technology and Therapy, Trans-Ekulu, P.M.B. 01473, Enugu, Nigeria.

Ifeanyichukwu Romanus Iroha, Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, P.M.B. 53, Nigeria

Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, P.M.B. 53, Nigeria

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Published

15-09-2023

How to Cite

John-Onwe , B. N. ., Ibiam, F. A. ., Udenweze, E. C. ., Iroha, C. S. ., Edemekong, C. I. ., Peter, I. U. ., & Iroha, I. R. . (2023). Co-expression of Extensively drug resistant (XDR) clinical isolates of Pseudomonas aeruginosa harboring FOX and MOX ampicillinase Gene. International Journal of Medical Sciences and Pharma Research, 9(3), 14–19. https://doi.org/10.22270/ijmspr.v9i3.76